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An integrated on-line SPE-HPLC-MS/MS system has been developed for rapid analysis ofvarious groups of perfluorinated compounds (PFCs) in surface and drinking water. 17 PFCs wereincluded in this study, with various carboxylic, sulfonate, and sulfonamide species among them.Use of Lower Pressure Turbulent-flow chromatography columns (LP-TFC) as solid phaseextraction (SPE) cartridges enables fast on-line SPE at high sampling flow-rate (3-5 ml/min).Polymeric Oasis hydrophile-lipophile balance (HLB) LPTFC column allow complete extraction with good recoveries frompreserved water. On-line coupling to high pressure liquid chromatography (HPLC) is performed with re-mixing of the organic LPTFCeluate with water in front of the analytical column to ensure efficient band focusing or a rapidincrease and decrease of organic solvent gradient can be used instead for efficient band focusing.For fast HPLC analysis, a short C18 column with 1.8 m particle size is applied in combinationwith highly selective API-MS/MS detection. Matrix effects on electro spray ES-MS-/MS signalwere found to be adequately corrected by using isotopic labeled standards and or using standardaddition technique. Limits of detection, determined for 1-ml sample of source water were in therange between 1 to 5ng/L typically for carboxylic and sulfonate PFC groups with chain length ofC4-C9, and about 20ng/L for sulfonamide and C10-C14 PFCs. At an enriched water volume of1 ml, the whole SPE-HPLC-MS/MS procedure requires less than 10 minutes. The method wassuccessfully applied to the analysis of drinking and source water samples for most of PFCs, thelonger chain PFCs C10-C14 needs further refinements. Includes 15 references, tables, figures.

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Edition: Vol. - No. Published: 11/01/2007 Number of Pages: 16File Size: 1 file , 570 KB