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AWWA WQTC62599

AWWA WQTC62599 Statistical Model to Assess False Negative PCR Reactions from Water Matrices

Conference Proceeding by American Water Works Association, 11/01/2005

Wolk, Donna M.; Hoffman, R.; Sturbaum, G.; Plummer, C.; Sterling, C.; Marshall, M.

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This slide presentation outlines a statistical model for assessing false negative polymerase chain reactions (PCR) from water matrices. Topics covered include: background on the microsporida organism; potential for environmental transmission; recommendation for human testing; molecular methods for microsporidia; experimental design of the AWWARF #2901, ENC-PCR; assay optimization, extraction methods and sample volume; specificity, virtual and actual; calculated efficiency; trend analysis; monitoring inhibitors in matrix; potential use for statistical comparison of IC-Ct; creating a statistical model; source water testing; and, counteracting inhibitors. Includes tables, figures.

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