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Successful and stable operation of a biological bromate reduction process is more likely whenthe bromate-reducing microbial community has been thoroughly characterized. In this study, theobjective was to characterize bromate reduction at the microbial level using eight bromate-reducingisolates from groundwater. Specific project objectives were to:verify bromate reduction to bromide by the isolates;investigate the reduction of nitrate by the bromate-reducing isolates;sequence the 16S rDNA of the bromate-reducing isolates; and,investigate bioaugmentation with the bromate-reducing isolates to shorten the start-uptime of a new BAC filtration process for bromate reduction.Isolation of bromate-reducing microorganismsM-R2A agar plates, containing essential nutrients, 100 g/L bromate, and 1 mM nitrate, wereprepared and degassed in an anaerobic chamber. The plates were inoculated with dilutedbiomass from a 3-year-old BAC filter that had treated Champaign-Urbana tapwatersupplemented with 20 g/L bromate. The plates were incubated at room temperature in an argonatmosphere. Various colonies were streaked to purity and tested for bromate reduction inaqueous medium. Includes 5 references.

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Edition: Vol. - No. Published: 11/01/2005 Number of Pages: 4File Size: 1 file , 160 KB