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The main objective of this study was to characterize the effects of water quality parameterson the inactivation kinetics of A.hydrophila (ATCC 7966) with monochloramine.An additional objective was to characterize the effect of dynamic disinfection on the inactivationkinetics of A. hydrophila with monochloramine. Variability in the inactivation kinetics ofA. hydrophila observed in this study is also addressed.Preparation of bacterial suspension. A. hydrophila (ATCC 7966) in freezed-dried formwas obtained from ATCC (American Type Culture Collection, Manassas, Virginia).Experimental procedures to recover the bacterial pellet and to prepare the bacterialsuspension for disinfection experiments were described previously (Sirikanchana et al.,2002) with some exceptions. Bacterial suspension in the total volume of 25 ml, ratherthan 49 ml, was incubated in a 50-mL centrifuge tube at 30C and placed in a shakingincubator for 25 hours instead of 41.5 hours. Sterile glycerol was mixed with the bacterialsuspension to obtain a final concentration of 25% (v/v) rather than 10% (v/v).The effect of water temperature on the inactivation kinetics of A.hydrophila with monochloramine was characterized by performing disinfectionexperiments at 5-30C. The experiments were performed with batch reactors immersed ina recirculating water bath. Synthetic solutions were used in this study. The pH wasmaintained constantly at pH 6 or 8 using 0.01 M phosphate buffer, or pH 10 using 0.01 Mborate buffer. Monochloramine concentrations investigated ranged from 0.5 to 5 mg/L asCl2.Experimental procedures formonochloramine disinfection and viability assessment followed the instruction asdescribed by Sirikanchana et al. (2002). Includes 5 references, figure.

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Edition: Vol. - No. Published: 11/02/2003 Number of Pages: 4File Size: 1 file , 260 KB