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The objective of this study was to develop the Random Amplified Polymorphic DNA (RAPD)technique to genotype Escherichia coli. The method gave results in accordance withribotyping and PFGE for nineteen strains and appeared to be more discriminative thanribotyping and less than PFGE. The Random Amplified Polymorphic DNA (RAPD)technique was used to genotype Escherichia coli strains isolated after a storm event and toprovide information on the origin of the bacterial contamination of the water intake of awater treatment plant which produces drinking water from river water among several wastewater outlets located upstream. A total of 650 strains were characterized using the RAPDtechnique. The E. coli population of the water intake appeared to be very heterogeneous andonly one third of this population could be retrieved from the four outlets. The remainingpopulation probably originated from some other effluents or from re-suspension ofsediments during rain events. The RAPD typing method applied in this study appeared rapid,robust and sensitive and proved to be well suited for the characterization of large bacterialenvironmental populations. Includes 28 references, tables, figure.

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Edition: Vol. - No. Published: 11/02/2003 Number of Pages: 11File Size: 1 file , 320 KB