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This research investigated a novel procedure using microsatellite-telomereprimers with PCR and PAGE to differentiate Cryptosporidium isolated from samples ofthree separate human groups in Australia and England, and to domestic sheep and cattle,to feral goats, and to kangaroo populations in three regions of Australia. Typing resultshave shown that the procedure can distinguish both common-origin and diverse origintypes in groups of common contact humans ranging from 10 to 20 individuals. Bothcommon and diverse types have been found in domestic sheep, feral goats, and kangaroossharing the same grazing area. A single stable type has been found among representativesof all infected calves of a large dairy over a period of 3-years. Diverse types have beenfound among dairy calves at three different dairies within a 50-mile radius in one area ofsoutheastern Australia. In summary, application of this procedure has revealed significantdiversity among C. parvum samples isolated from naturally infected hosts in both humanand animal populations. The findings suggest that general application of the procedurewill provide for sensitive source tracking and molecular epidemiological investigation. Includes 5 references, table, figures.

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Edition: Vol. - No. Published: 11/02/2003 Number of Pages: 6File Size: 1 file , 560 KB