AWWA WQTC57104

AWWA WQTC57104 Cryptosporidium and Giardia Detection and Discrimination of Cryptosporidium parvum Genotypes by Real-Time PCR
Conference Proceeding by American Water Works Association, 11/01/2002

Tanriverdi, Sultan; Tanyeli, Atilla; Koksal, Fatih; Baslamisli, Fikri; Kilinc

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This study designed real-time PCR assays which amplify Cryptosporidium parvum sequences with multiple nucleotide differences between type 1 and type 2. The presence of single-nucleotide polymorphisms affected the amplicon melting temperature and the melting temperature of internal fluorescent probes, the basis for a type-specific diagnostic assay. By combining two pairs of PCR primers, one specific for Cryptosporidium parvum the other for Giardia lamblia, a multiplex assay for detecting both organisms was developed. Includes 17 references, table, figures.