AWWA PATH56521

AWWA PATH56521 Identification and Characterization of Infectious and Non-infectious Sub-Populations of Encephalitizoon intestinalis Spores Purified from in vitro Cell Culture
Conference Proceeding by American Water Works Association, 09/22/2002

Marshall, Marilyn M.; Hoffman, Becky; Moffett, Jackie; Jost, B. Helen; Ware, Mike; Sturbaum, Greg; Battigelli, David

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This report describes flow cytometric identification, cell and molecular characterization of two E. intestinalis subpopulations isolated from cell culture propagated, Percoll-purified spore suspensions. The results of the molecular analysis of both spore subpopulations indicated that both populations possessed E. intestinalis 16S rRNA and ¿-tubulin genes. However, only one subpopulation was able to infect RK-13 cells. Researchers should be aware that there is inherent variability in the viability of microsporidia spores propagated using cell culture. Includes 11 references.