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Drinking water quality assessment involves the specific, sensitive and rapid detection of bacterial indicators in water samples. This work assesses the sensitivity of a new FISH method, combined with a physiological assay for direct enumeration at single-cell level of highly diluted viable Enterobacteriaceae cells in drinking waters. The approach (DVC-FISH) was completed by the use of a new direct detection device, the solid-phase laser cytometer (ScanRDI). The DVC-FISH method combined with ScanRDI detection make it possible to detect as little as one targeted cell in approximately 10(8) no targeted cells plotted on a membrane. This new approach as applied to a drinking water distribution system was investigated in this study. Includes 12 references, table, figures.

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Edition: Vol. - No. Published: 01/01/2001 Number of Pages: 13File Size: 1 file , 360 KB