AWWA WQTC50427

AWWA WQTC50427 Quantitation of Intact and Infectious Cryptosporidium Parvum Oocysts Using Quantitative Sequence Detection (QSD)
Conference Proceeding by American Water Works Association, 01/01/1999

Di Giovanni, George D.;Denhart, Mark;LeChevallier, Mark W.;Abbaszadegan, Morteza

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In this study quantitative sequence detection (QSD) primers and fluorogenic probes were evaluated using recombinant plasmid DNA containing the C. parvum hsp70 target and C. parvum oocysts DNA. CC-QSD was then used as a quantitative cell culture infectivity assay to evaluate the effects of water sample matrices on the potassium permanganate (KMnO4) and chlorine dioxide (ClO2) inactivation of C. parvum. Methods used include: oocysts and microscopy; oocyst treatments; in vitro cell culture of C. parvum; sample preparation for QSD and CC-QSD; and, QSD and CC-QSD. Includes 9 references, table, figures.