Full Description

This powerpoint presentation begins by providing background information on: coliform bacteria, water quality and safety;E. coli and coliforms, indicators of potential faecal contamination; absence of indicator bacteria, does not guarantee absence of pathogens; and,eight commonly used methods in Australian water testinglaboratories for the detection of coliform bacteria. The objective of this study was to evaluate the eight enumeration methods routinely used in watertesting laboratories for the detection of coliform bacteria in watersamples using a precise number of bacteria. Materials and methods included:BioBall vials containing freeze-dried E. coli and E. aerogenescultures; testing all methods 10 times with each BioBall culture; the mean and standard deviation of all readings was calculated; and,three different batches of BioBall were used. Conclusions were that:for methods enumerating 1 ml samples (Petrifilm and SPC), Petrifilmand PCA-P produced the most accurate and consistent results;for methods enumerating 100 ml samples (MF, DST and MPN),Colilert produced the most consistent results;MF, Colisure and MPN produced results with greater variability thanfirst expected; and,BioBall is a very suitable quality control for evaluating the efficiencyof water testing procedures. Includes figures.

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Edition: Vol. - No. Published: 11/01/2007 Number of Pages: 26File Size: 1 file , 2 MB